Composite

Part:BBa_K1695046:Design

Designed by: Aruna Surendran Ramkrishnan   Group: iGEM15_CityU_HK   (2015-08-17)


pLacIQ + RBS Bacteriophage 21 Codon Optimized Lysis Cassette S R Rz


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1760
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1312
    Illegal AgeI site found at 1605
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

With LacI promoter


Source

E.coli K12 JM109

References